Detection of Air-borne Pasteurella tularensis Using the Fluorescent Antibody Technique

Detection of air-borne Pasteurella tularensis using the fluorescent antibody technique.

Considerable research has been directed toward the development of rapid methods for the identification of air-borne microorganisms. The application of the fluorescent antibody technique (FAT) coupled with the impaction of contaminated air onto glass slides affords a rapid and specific method for the identification of air-borne Pasteurella tularensis. Early experiments presented problems of cros...

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Preparation of agglutinating antisera and fluorescent-antibody conjugates against Pasteurella tularensis in equines.

The serological response in burros and horses to the viable LVS strain of Pasteurella tularensis was studied. High-titered agglutinating antisera and fluorescent-antibody conjugates were obtained in both groups of animals. Maximum titers were obtained in horses 14 to 21 days after the start of vaccination and in burros 21 to 28 days after the start of vaccination. The use of Woodhour's adjuvant...

Rapid detection and identification of Streptococcus iniae using a monoclonal antibody-based indirect fluorescent antibody technique

Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems. The traditional plate culture technique to detect and identify S. iniae is time consuming and may be problematic due to phenotypic variations of S. iniae isolates. The rapid transmission and severe economic impact of this disease necessita...

Succinoxidase system of Pasteurella tularensis.

Preparation of washed particles. Cell-free extracts of highly virulent P. tularensis strain Sm were prepared by sonic disintegration of cells as described by Rendina and AMills (1957). Twenty ml of undialyzed centrifuged (3000 x G for 30 min) sonic extract, containing 3.8 to 4.2 mg SN per ml, were centrifuged for 3 hr at 105,000 X G at 5 C. The supernatant was decanted and thc reddish precipita...